CaCo-2 cells (kindly provided by Konstantin Sparrer, University Hospital Ulm, Germany) were cultivated in DMEM (Dulbecco’s Modified Eagle Medium DMEM; 11500516, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS-12A; Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM Gluta-MAX™ (35050061, Thermo Fisher Scientific), 25 mM HEPES (15630080, Thermo Fisher Scientific), 1× MEM Non-Essential Amino Acids Solution (11140050, Thermo Fisher Scientific), and 50 µg/mL gentamycin (1405-41-0, Serva Electrophoresis, Heidelberg, Germany) and passaged every 2–3 days depending on confluence.
HEK293T T7/N cells (previously described in [19 (link)]) were cultivated in DMEM (Dulbecco’s Modified Eagle Medium DMEM; 11500516, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated FBS, 2 mM Gluta-MAX™, 25 mM HEPES, 5 µg/mL blasticidin (asnt-bl-1, InvivoGen, San Diego, CA, USA), and 2 µg/mL puromycin (SC-1080713, Santa Cruz Biotechnology, Dallas, TX, USA).
All cells were incubated at 37 °C, 5% CO2, and 80% relative humidity.
HEK293T T7/N cells (previously described in [19 (link)]) were cultivated in DMEM (Dulbecco’s Modified Eagle Medium DMEM; 11500516, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated FBS, 2 mM Gluta-MAX™, 25 mM HEPES, 5 µg/mL blasticidin (asnt-bl-1, InvivoGen, San Diego, CA, USA), and 2 µg/mL puromycin (SC-1080713, Santa Cruz Biotechnology, Dallas, TX, USA).
All cells were incubated at 37 °C, 5% CO2, and 80% relative humidity.
Free full text: Click here
