Blood was obtained by puncture of the retro-orbital plexus. Anticoagulation was performed with 0.1 volume of 136 mmol/L trisodium citrate. Subsequently, plasma was immediately isolated by centrifugation at 1100× g for 10 min and stored at –20 °C [54 (link),55 (link),56 (link)].
Plasma cholesterol and lipoprotein cholesterol levels in C57BL/6J LDLr−/− mice were determined using the Cholesterol Quantification kit from Sigma-Aldrich (Sigma, St. Louis, MO, USA). HDL and non-HDL lipoproteins were separated by ultracentrifugation as described [12 (link)]. Plasma triglyceride concentration was quantified using the Triglyceride Quantification Kit MAK266 (Sigma-Aldrich) according to the instructions of the manufacturer.
Plasma cholesterol and lipoprotein cholesterol levels in C57BL/6J LDLr−/− mice were determined using the Cholesterol Quantification kit from Sigma-Aldrich (Sigma, St. Louis, MO, USA). HDL and non-HDL lipoproteins were separated by ultracentrifugation as described [12 (link)]. Plasma triglyceride concentration was quantified using the Triglyceride Quantification Kit MAK266 (Sigma-Aldrich) according to the instructions of the manufacturer.
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