Culturing HUH6 and HB-243 Hepatoblastoma Cell Lines

HB cell line HUH6 was obtained from Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM)-glutaMAX (Gibco) supplemented with 10% FBS (Gibco), 100 U/mL penicillin (Gibco), and 100 μg/mL streptomycin sulfate (Gibco, Waltham, MA, United States). HB cell line HB-243 from patient-derived xenograft (PDX) was provided by XenTech (Evry, France) (Kats et al., 2019 (link)). HB-243 cells were cultured in Advanced DMEM/F12 (Gibco, Waltham, MA, United States) supplemented with 8% fetal bovine serum (FBS) (Gibco), 2 mM glutaMAX (Gibco), 100 U/mL penicillin (Gibco), 100 μg/mL streptomycin sulfate (Gibco) and 20 μM rock kinase inhibitor Y-27632 (S1049; SelleckChem, Houston, TX, United States). Absence of mycoplasma was regularly confirmed with PCR-based method (PromoCell, Heidelberg, Germany).

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Nousiainen R., Eloranta K., Isoaho N., Cairo S., Wilson D.B., Heikinheimo M, & Pihlajoki M. (2023). UBE2C expression is elevated in hepatoblastoma and correlates with inferior patient survival. Frontiers in Genetics, 14, 1170940.

Publication 2023

DMEM)-glutaMAX FBS penicillin streptomycin sulfate Advanced DMEM/F12 glutaMAX Y-27632

Cell line Cells Cultured medium Eagle Fetal bovine serum Japanese Kinase Mycoplasma Patient Penicillin Streptomycin sulfate Xenograft Y 27632

Corresponding Organization :

Other organizations : Statistics Sweden

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Variable analysis

independent variables

Cell line used (HUH6 and HB-243)

dependent variables

Not explicitly mentioned

control variables

Media composition (DMEM-glutaMAX, Advanced DMEM/F12)
Supplements (10% FBS, 8% FBS, 2 mM glutaMAX, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, 20 μM rock kinase inhibitor Y-27632)
Absence of mycoplasma confirmed by PCR-based method

controls

Positive control: Not specified
Negative control: Not specified

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