Induction of adipogenic differentiation was performed using an adipogenic differentiation medium kit (Cyagen Biosciences Inc., China) as previously reported [7 (link)]. UC-MSCs were seeded in six-well plates and treated with adipogenic medium for 21 days, with the medium changed 3 times per week. Adipogenesis was assessed by Oil Red O staining.
To induce osteogenic differentiation, UC-MSCs were seeded in gelatin-coated six-well plates and treated with osteogenic medium (Cyagen Biosciences Inc., China) for 21 days, with the medium changed 3 times per week as previously described [8 (link)]. Osteogenesis was assessed by alizarin red staining. All photos were taken under an Olympus CKX53 microscope.
To induce osteogenic differentiation, UC-MSCs were seeded in gelatin-coated six-well plates and treated with osteogenic medium (Cyagen Biosciences Inc., China) for 21 days, with the medium changed 3 times per week as previously described [8 (link)]. Osteogenesis was assessed by alizarin red staining. All photos were taken under an Olympus CKX53 microscope.
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