T Cell Activation and MDSC Co-culture
Corresponding Organization :
Other organizations : The University of Texas MD Anderson Cancer Center, Baylor College of Medicine, Texas A&M Health Science Center, University of Houston, Wayne State University, The University of Texas Health Science Center at Houston
Variable analysis
- T cells were isolated from the spleens of wildtype BALB/c mice
- T cells were stained with eFluor 450
- T cells were activated by culturing in the presence of 3 mg/ml of anti-CD3 antibody (BioLegend, catalogue number 100302) and 1 mg/ml of anti-CD28 antibody (BioLegend, catalogue number 102102)
- CD8+ T-cell proliferation
- Cells were then incubated with MDSCs for 4 days at 37 °C
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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