T cells were isolated from the spleens of wildtype BALB/c mice, stained with eFluor 450 and activated by culturing in the presence of 3 mg/ml of anti-CD3 antibody (BioLegend, catalogue number 100302) and 1 mg/ml of anti-CD28 antibody (BioLegend, catalogue number 102102) as previously described.38 (link) Cells were then incubated with MDSCs for 4 days at 37 °C. CD8+ T-cell proliferation was then analysed.
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