Cultivation and Genome Sequencing of Botrytis cinerea

Strain B05.10 of B. cinerea Persoon:Fries is an isolate from Vitis vinifera (Table 1) and is used as recipient strain for genetic modifications. The genome sequence of B05.10 was published [50] (link) and recently updated by Staats and van Kan [51] (link). B. cinerea strains were cultivated on plates containing solid synthetic complete medium (CM) [52] (link), Gamborg B5 ( = GB5) +2% glucose (Duchefa, The Netherlands), modified Czapek-Dox (CD) as minimal medium (2% sucrose, 0.1% KH₂PO₄, 0.3% NaNO₃, 0.05% KCl, 0.05% MgSO₄ x 7 H₂O, 0.002% FeSO₄ x 7 H₂O, pH 5.0), or solid potato dextrose (PDA) agar (Sigma-Aldrich, Germany) supplemented with 10% homogenized bean leaves (PDAB). Cultures were incubated at 20°C under white light (12 h light/12 h darkness) for conidiation, and in continuous darkness for induction of sclerotia formation. For DNA isolation, mycelia were grown on solid CM with cellophane overlays.