The human oral keratinocyte cell line OKF6/TERT1 was grown in keratinocyte serum free medium (KSFM) supplemented with L-glutamine and penicillin-streptomycin-fungizone (Sigma-Aldrich) in the presence of 0.03 M calcium chloride and bovine pituitary extract as described previously11 (link). Primary cultures of normal human gingival epithelial cells were grown as previously described19 (link),20 (link).
Protocol detail
Culturing Oral Keratinocyte Cell Lines
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Bovine
Calcium chloride
Cell line
Fungizone
Gingival
Glutamine
Human
Keratinocyte
Normal cells cultures
Penicillin
Serum
Streptomycin
Corresponding Organization : Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- Presence of 0.03 M calcium chloride
- Presence of bovine pituitary extract
- Growth of OKF6/TERT1 cell line
- Growth of primary cultures of normal human gingival epithelial cells
- Keratinocyte serum free medium (KSFM)
- L-glutamine
- Penicillin-streptomycin-fungizone (Sigma-Aldrich)
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