Quantifying Salmonella and Lactobacillus in Caeca

The contents of paired caeca were collected and frozen at −20 °C for DNA extraction and Salmonella and Lactobacillus quantification by real-time PCR. DNA from caecal samples was extracted using a QIAamp Stool kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). Based on known genomic sequences, strain-specific real-time PCRs were designed to determine colonisation of the caecum by S. Enteritidis and each of the lactobacilli isolates (Table 1). Real-time PCR in SybrGreen format was performed exactly as described previously [26 (link)].

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Juricova H., Matiasovicova J., Faldynova M., Sebkova A., Kubasova T., Prikrylova H., Karasova D., Crhanova M., Havlickova H, & Rychlik I. (2022). Probiotic Lactobacilli Do Not Protect Chickens against Salmonella Enteritidis Infection by Competitive Exclusion in the Intestinal Tract but in Feed, Outside the Chicken Host. Microorganisms, 10(2), 219.

Publication 2022

QIAamp Stool kit

Based sequences Caecum Frozen Genomic Lactobacilli Real time pcr Salmonella Stool Strain

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Salmonella colonization of the caecum
Lactobacillus colonization of the caecum

control variables

DNA extraction using QIAamp Stool kit according to manufacturer's instructions
Real-time PCR in SybrGreen format as described previously [26]

controls

Positive control: Not specified
Negative control: Not specified

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