Quantification of Tissue Iron Levels

Plasma iron was measured using QuantiChrom Iron Assay kit (BioAssay Systems, Hayward, CA, USA) according to the manufacturer’s instructions. Tissue iron determination was performed as described [27 (link),36 (link),40 (link)]. After acidic hydrolysis at 65 °C for 24 h, the iron content was measured using a colorimetric staining solution containing sodium acetate and bathophenanthroline disulfonic acid. Total tissue iron content was normalized by protein content.

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Fischer C., Valente de Souza L., Komlódi T., Garcia-Souza L.F., Volani C., Tymoszuk P., Demetz E., Seifert M., Auer K., Hilbe R., Brigo N., Petzer V., Asshoff M., Gnaiger E, & Weiss G. (2022). Mitochondrial Respiration in Response to Iron Deficiency Anemia: Comparison of Peripheral Blood Mononuclear Cells and Liver. Metabolites, 12(3), 270.

Publication 2022

QuantiChrom Iron Assay kit

Acidic Bathophenanthroline disulfonic acid Bioassay Colorimetric Hydrolysis Iron Plasma Protein Sodium acetate Tissue

Corresponding Organization : Innsbruck Medical University

Other organizations : Oroboros Instruments (Austria)

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma iron content
Tissue iron content

control variables

Protein content (used to normalize total tissue iron content)

controls

Positive control: None mentioned
Negative control: None mentioned

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