Three human TSCC cell lines, SCC-9, CAL-27 and SCC-15, as well as normal gingival epithelial cells (ATCC® PCS-200-014™) were purchased from the American Type Culture Collection (ATCC). Previous studies (26 (link),27 (link)) have used the normal gingival epithelial cells as a control for TSCC cell lines. Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution (all Invitrogen; Thermo Fisher Scientific, Inc.) was utilized for cell culture. All cells were maintained in a humidified incubator at 5% CO2 and 37°C.
Protocol detail
Culturing TSCC Cell Lines and Normal Gingival Cells
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CAL-27
SCC-15
ATCC® PCS-200-014™)
Dulbecco's modified Eagle's medium (DMEM)
penicillin/streptomycin solution
Cell culture
Cell lines
Cells
Epithelial cells
Fetal bovine serum
Gingival
Human
Penicillin
Streptomycin
Corresponding Organization :
Other organizations : Shengli Oilfield Central Hospital
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Variable analysis
independent variables
- Three human TSCC cell lines: SCC-9, CAL-27, and SCC-15
- Unspecified
- Normal gingival epithelial cells (ATCC® PCS-200-014™)
- Normal gingival epithelial cells (ATCC® PCS-200-014™) were used as a control for the TSCC cell lines, as mentioned in previous studies (26, 27).
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