GC-MS Analysis of Derivatized Lipids

Derivatized lipids were separated with an Agilent 7890B Series GC equipped with 2 Agilent DB-17HT columns in tandem (each 30 m x 0.25 mm i.d. x 0.15 μm film thickness) with helium as the carrier gas at a constant flow of 1.1 ml/min. The program was as follows: 100 °C for 2 min, then 12 °C/min to 250 °C and held for 10 min, then 10 °C/min to 330 °C and held for 17.5 min. Two µL of each sample was injected in splitless mode at 250 °C. The GC was coupled to an Agilent 5977 A Series MSD with the ion source at 230 °C and operated at 70 eV in EI mode scanning from 50 to 850 Da in 0.5 s. Lipids were identified based on their retention time and compared to previously published spectra (Wei et al., 2016 (link)).