Fluorescence Imaging of Intestinal Tissue

Fluorescence imaging was performed using a multimodal IVIS Lumina XR imaging system (PerkinElmer). The mice were euthanised by cervical dislocation, followed by exposure of the abdominal cavity and removal of the intestine from the stomach to the rectum [33 (link)]. The instrument background fluorescence was removed using an adaptive fluorescence background subtraction tool. Images were analysed using Living Image version 4.3.1 [33 (link)].

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Zhang Y., Yang L., Zhang J., Huang K., Sun X., Yang Y., Wang T., Zhang Q., Zou Z, & Jin M. (2022). Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus. Microbial Cell Factories, 21, 185.

Publication 2022

IVIS Lumina XR

Abdominal cavity Adaptive Cervical Dislocation Fluorescence Intestine Mice Rectum Stomach

Corresponding Organization :

Other organizations : Huazhong Agricultural University

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Variable analysis

independent variables

Fluorescence imaging

dependent variables

Fluorescence intensity

control variables

Instrument background fluorescence

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