Pilocarpine-Induced Status Epilepticus in Mice

We created a C57/BL6 male mouse SE model, as previously described^{30 (link)}. Seven-week-old mice received 2 mg/kg of scopolamine methyl bromide (intraperitoneally [i.p.]; S8502; Sigma-Aldrich, St. Louis, MO, USA) and terbutaline hemisulfate salt (2 mg/kg, i.p.; T2528; Sigma-Aldrich) to block the peripheral effects of pilocarpine and dilate the respiratory tract to reduce mortality, respectively. After 30 min, the muscarinic agonist pilocarpine hydrochloride (320 mg/kg, freshly prepared, i.p.; P6503; Sigma-Aldrich) was injected to induce SE. Seizure activity was scored using Racine’s classification^{31 (link)}. Only mice with a Racine’s scale stage of 5 or above for behavioral seizures were selected for the experiments.