MosSCI transformation was performed based on the protocol described in [3] (link)
(http://sites.google.com/site/jorgensenmossci/ ). The Mos1
insertion strains EG4322 or EG5003 were used for injection. Injection mixes
contained pJL43.1 (50 ng/µl), pCJF90 (2.5 ng/µl), pCFJ104 (5
ng/µl), and the respective expression clone (50 ng/µl) in 20 mM
potassium phosphate and 3 mM potassium citrate (pH 7.5). We note that although
we were able to obtain transgenic strains expressing each of the constructs
described, some apparent integration events did not result in detectable
expression; we do not know the reason for this variability.
(
insertion strains EG4322 or EG5003 were used for injection. Injection mixes
contained pJL43.1 (50 ng/µl), pCJF90 (2.5 ng/µl), pCFJ104 (5
ng/µl), and the respective expression clone (50 ng/µl) in 20 mM
potassium phosphate and 3 mM potassium citrate (pH 7.5). We note that although
we were able to obtain transgenic strains expressing each of the constructs
described, some apparent integration events did not result in detectable
expression; we do not know the reason for this variability.
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