We have studied how the latrunculin B treatment can influence the cellular force generation. For this purpose, ~1×106/mL HeLa cells were first pretreated with 5–60 μM of latrunculin B for 60 min at 37 °C inside a cell culture incubator. After that, cells were separated and dispersed in cell culture media, ~1×105 cells was then casted onto the surface of the TGT sensor and incubated for 75 min. After washing with 0.1 M phosphate buffer (pH 7.4) for several times, the SWV of 5 mM [Fe(CN)6]4− on the electrode was recorded using the above-mentioned parameters: step potential, 20 mV; pulse amplitude, 50 mV; and frequency, 20 Hz.
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Latrunculin B Influences Cellular Force
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Other organizations : University of Massachusetts Amherst
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Variable analysis
independent variables
- Latrunculin B concentration (5-60 μM)
- Cellular force generation
- Cell type (HeLa cells)
- Cell density (1×10^6/mL)
- Incubation time (60 min)
- Temperature (37°C)
- Electrode parameters (step potential: 20 mV, pulse amplitude: 50 mV, frequency: 20 Hz)
- Electrolyte concentration (5 mM [Fe(CN)6]4-)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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