At Wave 1, a venous blood sample (25 mL) was collected from each participant who provided consent. Samples were transported to a central laboratory in temperature-controlled shipping boxes where they were centrifuged, aliquoted into cryovials and stored at −80°C. Cystatin C and creatinine were measured simultaneously from frozen plasma. Cystatin C was measured using a second-generation particle-enhanced immunoturbidimetric assay (Roche Tina-quant) on a Roche Cobas 701 analyser. The assay has a measuring range of 0.40–6.80 mg/L and is traceable to the European reference standard material (ERM-DA471/IFCC) for cystatin C. Creatinine was measured on the same analyser using an enzymatic method traceable to isotope-dilution mass spectrometry. GFR was estimated using the Chronic Kidney Disease Epidemiology Collaboration equations for cystatin C [18 (link)] or creatinine [19 (link)].
Protocol detail
Evaluating Kidney Function using Cystatin C and Creatinine
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Assay
Chronic kidney disease
Creatinine
Cystatin c
Dilution
Enzymatic
European
Frozen
Immunoturbidimetric assay
Isotope
Mass spectrometry
Plasma
Tina
Venous blood
Corresponding Organization : Trinity College Dublin
Other organizations : Royal College of Surgeons in Ireland, Beaumont Hospital
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Variable analysis
independent variables
- Cystatin C measurement method
- Creatinine measurement method
- Cystatin C concentration
- Creatinine concentration
- Estimated glomerular filtration rate (eGFR) based on cystatin C
- Estimated glomerular filtration rate (eGFR) based on creatinine
- Volume of venous blood sample (25 mL)
- Centrifugation and aliquoting of samples
- Storage temperature (-80°C)
- Assay measuring range for cystatin C (0.40–6.80 mg/L)
- Traceability of cystatin C assay to European reference standard material (ERM-DA471/IFCC)
- Traceability of creatinine assay to isotope-dilution mass spectrometry
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