miRNA Microarray Analysis Protocol

Total RNA (100 ng) was hybridized on an Agilent 8x15K human miRNA microarray, following the manufacturer’s instructions (Agilent Technologies) [30 (link)]. Scanning was carried out immediately using the Agilent G2565AA Microarray Scanner System (Agilent Technologies) and data were collected with Feature Extraction v9.5 software (Agilent Technologies). Significant miRNAs (p<0.05) were represented by a cluster using Babelomics 4.2 software (http://babelomics.bioinfo.cipf.es). The microarray is available at the Gene Expression Omnibus under accession number GSE109421.

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Manso R., Martínez-Magunacelaya N., Eraña-Tomás I., Monsalvez V., Rodríguez-Peralto J.L., Ortiz-Romero P.L., Santonja C., Cristóbal I., Piris M.A, & Rodríguez-Pinilla S.M. (2018). Mycosis fungoides progression could be regulated by microRNAs. PLoS ONE, 13(6), e0198477.

Publication 2018

Feature Extraction v9.5 software

Gene expression Human Microarray Mirnas

Corresponding Organization : Hospital Universitario 12 De Octubre

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Variable analysis

independent variables

Total RNA (100 ng)

dependent variables

MiRNA expression levels

control variables

Agilent 8x15K human miRNA microarray
Agilent G2565AA Microarray Scanner System
Feature Extraction v9.5 software
Babelomics 4.2 software

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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