Evaluating Cellular Responses to Res Treatment

Hematoxylin-Eosin (H/E) staining was performed on cell-bearing coverslips to observe the morphological changes of Res-treated THJ-16T, THJ-21T and Nthy-ori 3-1 cells by the method described elsewhere 34 . 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay, EdU staining were performed to elucidate proliferative activity and trypan blue cell discrimination assay for cell death rates. Deoxynucleotidyl transferase-mediated dUTP-biotin nick and labeling assay (TUNEL, Beyotime Biotechnology, C1086, Shanghai, China) was used to analyze apoptotic cell death by the methods described previously 33 (link). The cell images were collected under a positive fluorescence microscope (Zeiss, Ax10 Axio, Germany).

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Xiong L., Nie J.H., Lin X.M., Wu J.B., Chen Z., Xu B, & Liu J. (2020). Biological implications of PTEN upregulation and altered sodium/iodide symporter intracellular distribution in resveratrol-suppressed anaplastic thyroid cancer cells. Journal of Cancer, 11(23), 6883-6891.

Publication 2020

Ax10 Axio

Apoptotic cell death Assay Biotin Bromide Cell Deoxynucleotidyl transferase Diphenyl Discrimination Dutp Eosin Fluorescence microscope Tetrazolium Trypan blue Tunel

Corresponding Organization : Guangzhou Medical University

Other organizations : First Affiliated Hospital of Guangzhou University of Chinese Medicine

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Variable analysis

independent variables

Res-treated

dependent variables

Morphological changes of THJ-16T, THJ-21T and Nthy-ori 3-1 cells
Proliferative activity
Cell death rates
Apoptotic cell death

control variables

Cell-bearing coverslips

controls

Positive control: Not specified
Negative control: Not specified

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