The LH surge was induced using an injection paradigm described in Bosch et al. [31 (link)]. Briefly, animals underwent bilateral ovariectomy. Five days following the procedure, animals were given a subcutaneous injection of 0.25 µg of β-estradiol (EB; catalog no. E8875; Sigma-Aldrich) in 100 µL of sesame oil (catalog no. S3547; Sigma-Aldrich) at ZT10. On the following day, the animals were given a subcutaneous injection of 1.5 µg of EB in sesame oil (100 µL) at ZT10. On the following day, 7 days after ovariectomy, the animals were euthanized at ZT4 (1000 hours) or ZT12 (lights off; 1800 hours) and blood was collected for LH measurement.
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Induction of LH Surge in Ovariectomized Rodents
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Corresponding Organization : University of California, San Diego
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Variable analysis
independent variables
- Injection of 0.25 µg of β-estradiol (EB) in 100 µL of sesame oil at ZT10 on day 5 after ovariectomy
- Injection of 1.5 µg of EB in 100 µL of sesame oil at ZT10 on the following day (day 6) after ovariectomy
- Luteinizing hormone (LH) measurement in collected blood samples
- Bilateral ovariectomy procedure performed 5 days prior to the first EB injection
- Euthanasia and blood collection at ZT4 (1000 hours) or ZT12 (1800 hours, lights off)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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