Sources and cultures of human normal bronchial epithelial BEAS-2B cells and alveolar type II epithelial A549 cells have been described in detail in our previous research [11 (link)]. IGF1R was silenced in 40 passages of AECs induced by CD-NPs (IGF1R-siRNA) with use of knockout lentiviruses according to the manufacturer’s instructions (Sangon Biotechnology Co., Ltd., Shanghai, China).
Picropodophyllin (PPP, AXL1717) (a selective IGF-1R inhibitor), MK2206 (a specific inhibitor of AKT) and TWS119 (a specific inhibitor of GSK3β) were procured from Selleck Chemicals (Houston, USA). Recombinant human IGF1 was obtained from PeproTech (New Jersey, USA). N-Acetylcysteine (NAC) (a ROS inhibitor) and MCC950 (a potent and selective NLRP3 inhibitor) were bought from MedChemExpress (New Jersey, USA).
Picropodophyllin (PPP, AXL1717) (a selective IGF-1R inhibitor), MK2206 (a specific inhibitor of AKT) and TWS119 (a specific inhibitor of GSK3β) were procured from Selleck Chemicals (Houston, USA). Recombinant human IGF1 was obtained from PeproTech (New Jersey, USA). N-Acetylcysteine (NAC) (a ROS inhibitor) and MCC950 (a potent and selective NLRP3 inhibitor) were bought from MedChemExpress (New Jersey, USA).
Free full text: Click here
