Immunization and Parasite Burden Analysis in Hamsters
Corresponding Organization :
Other organizations : The Ohio State University, McGill University, National Institute of Allergy and Infectious Diseases, Nagasaki University
Variable analysis
- Immunization of hamsters with 10^6 total stationary-phase LmexCen^-/- parasites by intradermal injection in the left ear
- Infection of age-matched control group with 10^6 total stationary-phase L. mexicana wild-type (LmexWT) promastigotes
- Lesion size measured weekly by the diameter of the ear lesion
- Parasite burdens in the ear, draining lymph node (dLN), spleen, liver, and bone marrow tissues determined by limiting dilution assay
- Six to eight-week-old female hamsters
- 10 μl PBS used for injections with a 29-gauge needle
- Liberase enzyme (0.15 mg/ml) in DMEM medium used to treat ear tissues
- M199 medium supplemented with 10% FBS and 1% Penicillin/Streptomycin used for cell suspension preparation
- Age-matched control group infected with 10^6 total stationary-phase L. mexicana wild-type (LmexWT) promastigotes
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