To isolate and enumerate the microorganisms listed below, ten grams were taken representative of each sample (SM and SR) and placed in sterile stomacher bag. Ninety milliliters of sterilized peptone water (PW; Oxoid, Madrid, Spain) were added to each bag (1:10, w/v), and then, the content was homogenized for three minutes at 230 rpm using a peristaltic homogenizer (BagMixer ® 400 P, Interscience, Saint Nom, France). Subsequently, ten-fold serial dilutions were prepared, and microorganisms were cultured as follows: (i) Total mesophilic aerobic bacterial counts (TAB 30 • C) were determined using plate count agar (PCA; Oxoid, Madrid, Spain) and incubated at 30 • C for 48-72 h [14] ; (ii) β-glucuron-positive Escherichia coli were isolated on Tryptone Bile x-Glucuronide (TBX; CM0945, Oxoid, Basingstoke, Hampshire, UK) and incubated at 44 • C for 24-48 h [15] ; (iii) Pseudomonas spp. were cultured on CFC agar (Cetrimide-Fucidin-Cephalothin agar with a modified CFC selective supplement, SR0103E; Oxoid, Basingstoke, UK) and incubated aerobically at 25 • C for 48 h [16] ; (iv) mesophilic lactic acid bacteria (LAB) were enumerated using De Man, Rogosa, and Sharpe agar (MRS, CM0361; Oxoid, Hampshire, UK) and incubated aerobically for 72 h at 30 • C [17] ; (v) yeasts and molds were detected on Dicloran Rose-Bengal Chloramphenicol Agar (DRBC; Oxoid, Madrid, Spain) incubated at 25 • C for 120-168 h [18] . After incubation and counting, the data were expressed as logarithms of the number of colony-forming units (Log CFU g -1 ), and the means and standard deviation were calculated.
Research of pathogenic bacteria was conducted to detect Listeria monocytogenes, Salmonella spp., Clostridium perfringens, and Bacillus cereus. To detect Listeria monocytogenes, 25 g of salami samples (SM and SR) were homogenized in 225 mL (1:10, w/v) of half Fraser broth (HFB, CM1053, Oxoid), spread on ALOA petri dishes (Agar Listeria according to Ottaviani and Agosti), and then incubated at 37 • C for 24 h [19] . For Salmonella spp., homogenization of 25 g of sample in 225 mL (1:10, w/v) of buffered peptone water (BPW; Oxoid, Madrid, Spain) was required, followed by transfer to Rappaport-Vassiliadis broth (RVS) and incubation at 41.5 • C for 24 h. Subsequently, the samples were plated on xylose lysine deoxycholate (XLD; Oxoid, Hampshire, UK) agar petri dishes and incubated at 37 • C for 24 h [20] . For Clostridium perfringens [21] and Bacillus cereus [22] , 1 mL of the solution prepared with peptone water (PW; Oxoid, Madrid, Spain) was inoculated on petri dishes and then incubated anaerobically at 37 • C for 18-24 h for both bacteria.
Research of pathogenic bacteria was conducted to detect Listeria monocytogenes, Salmonella spp., Clostridium perfringens, and Bacillus cereus. To detect Listeria monocytogenes, 25 g of salami samples (SM and SR) were homogenized in 225 mL (1:10, w/v) of half Fraser broth (HFB, CM1053, Oxoid), spread on ALOA petri dishes (Agar Listeria according to Ottaviani and Agosti), and then incubated at 37 • C for 24 h [19] . For Salmonella spp., homogenization of 25 g of sample in 225 mL (1:10, w/v) of buffered peptone water (BPW; Oxoid, Madrid, Spain) was required, followed by transfer to Rappaport-Vassiliadis broth (RVS) and incubation at 41.5 • C for 24 h. Subsequently, the samples were plated on xylose lysine deoxycholate (XLD; Oxoid, Hampshire, UK) agar petri dishes and incubated at 37 • C for 24 h [20] . For Clostridium perfringens [21] and Bacillus cereus [22] , 1 mL of the solution prepared with peptone water (PW; Oxoid, Madrid, Spain) was inoculated on petri dishes and then incubated anaerobically at 37 • C for 18-24 h for both bacteria.
