To examine different cell populations within human melanoma patient PBMC and mouse Pmel-1 splenocytes, cell surface flow cytometry was performed as previously described.27 (link) Human melanoma patient PBMC were stained with the following anti-human antibodies from BD: Fixable Viability Stain 575V, CD3 APC-R700, CD19 APC-H7, CD47 APC, PD-1 BV 421, and CD8 BUV496. Pmel-1 splenocytes were stained with dilutions of the following anti-mouse antibodies from BD: CD19 BV421, Fixable Viability Stain 575V, CD64 BV786, CD8a APC-H7, and CD3 BUV496. Flow cytometry was performed on the BD LSRFortessa X-20 Analyzer, and analysis was performed on FCS Express software (online supplemental figures 3C and 3D ).
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Multiparametric Flow Cytometry Analysis of Immune Cells
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Anti antibodies
Cd47
Cell
Dilutions
Flow cytometry
Human
Melanoma
Mouse
Patient
Pmel
Populations
Stain
Corresponding Organization : Atrium Health Wake Forest Baptist
Other organizations : National Cancer Institute
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Variable analysis
independent variables
- Human melanoma patient PBMC were stained with the following anti-human antibodies: Fixable Viability Stain 575V, CD3 APC-R700, CD19 APC-H7, CD47 APC, PD-1 BV 421, and CD8 BUV496
- Pmel-1 splenocytes were stained with dilutions of the following anti-mouse antibodies: CD19 BV421, Fixable Viability Stain 575V, CD64 BV786, CD8a APC-H7, and CD3 BUV496
- Examination of different cell populations within human melanoma patient PBMC and mouse Pmel-1 splenocytes
- Cell surface flow cytometry was performed as previously described.27
- No positive or negative controls were explicitly mentioned in the input.
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