Immunohistochemical Evaluation of MICAL1 in Renal Cancer

Renal cancer tissue microarrays were purchased from Outdo biotech (Shanghai, China). Immunohistochemical staining was performed as described previously [27 (link)]. Briefly, microarray tissues were dewaxed and incubated MICAL1 (Proteintech, Wuhan, China) and secondary antibody (Maxim Biotechnologies, Fuzhou, China) overnight at 4 °C. Following incubation with HRP-labelled secondary antibody, tissue sections were stained with DAB under microscopic observation and counterstained with hematoxylin. Typical images were captured under Olympus BX51 microscope. By evaluating the percentage of the number of stained cells and the staining intensity of MICAL1, the immunoreactivity score (IRS) was evaluated as described previously [28 (link)–30 (link)]. IRS was calculated as intensity of the staining reaction (0 to 3 points) multiplied by the percentage of positive cells (0 to 4 points).

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Yang Y., Ye F., Xia T., Wang Q, & Du J. (2022). High MICAL1 expression correlates with cancer progression and immune infiltration in renal clear cell carcinoma. BMC Cancer, 22, 1355.

Publication 2022

MICAL1 BX51 microscope

Antibody Cells Hematoxylin Microarray Microscope Renal cancer Tissue

Corresponding Organization :

Other organizations : Nanjing Medical University

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Variable analysis

independent variables

MICAL1 primary antibody (Proteintech, Wuhan, China)

dependent variables

Immunoreactivity score (IRS) of MICAL1 protein expression

control variables

Secondary antibody (Maxim Biotechnologies, Fuzhou, China)
Hematoxylin staining for counterstaining

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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