Healthy Asian Seabass Maintenance Protocol

Healthy Asian Seabass (2 ± 0.5g) were obtained from a commercial hatchery (Allegro Aqua, Singapore) and fish (n=120) were divided equally into four 200 L recirculating tanks each equipped with a standard biofiltration system and aeration at the experimental marine aquarium facilities of Temasek Life Sciences Laboratory (TLL, Singapore). Fish were maintained in 30 ± 1°C UV-irradiated seawater, with a 12:12 light and dark photoperiod cycle. All fish were fed with a commercial pellet diet at 5% body weight per day. During the acclimatization period, 5% of the fish were randomly sampled and analysed for NNV and bacterial diseases common to the species. Experimental animals were ascertained to be good health and tested negative for common seabass bacterial pathogens and betanodavirus prior to start of experiments. Animal experiments for this study were approved by the Institutional Animal Care and Use Committee (IACUC) of TLL (IACUC approval number TLL(F)-22-011).

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Chan J., Carmen L.C., Lee S.Q, & Prabakaran M. (2023). Identification and characterization of immunoglobulin tau (IgT) in Asian Seabass (Lates calcarifer) and mucosal immune response to nervous necrosis virus. Frontiers in Immunology, 14, 1146387.

Publication 2023

Acclimatization Allegro Asian Bacterial Bacterial diseases Betanodavirus Body weight Diet Experimental animals Fish Institutional animal care and use committee Light Marine Pathogens Seabass

Corresponding Organization : National University of Singapore

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

NNV and bacterial diseases common to the species

control variables

Healthy Asian Seabass (2 ± 0.5g)
Fish (n=120) divided equally into four 200 L recirculating tanks
Tanks equipped with a standard biofiltration system and aeration
Maintained in 30 ± 1°C UV-irradiated seawater
12:12 light and dark photoperiod cycle
Fed with a commercial pellet diet at 5% body weight per day
Acclimatization period with 5% of fish randomly sampled and analyzed for NNV and bacterial diseases

positive controls

None explicitly mentioned

negative controls

Experimental animals were ascertained to be in good health and tested negative for common seabass bacterial pathogens and betanodavirus prior to start of experiments

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