Olanzapine Effects on Lipid Metabolism

Olanzapine was purchased from the Tokyo Chemical Industry (Tokyo, Japan). The medium-fat diet (containing 1.37 IU of cholecalciferol/g) and cholecalciferol-supplemented medium-fat diet (containing 200 IU of cholecalciferol/g) were purchased from Oriental Yeast (Tokyo, Japan). Olanzapine was dissolved in water with 0.5% of carboxymethyl cellulose before use. Cholecalciferol is usually administered orally as a chow supplement, and the intake dose may vary slightly among mice. Mice were first randomized into two groups and fed a medium-fat diet or a cholecalciferol-supplemented medium-fat diet for 1 week. Mice from each group were further randomized to be treated with Olanzapine (10 mg/kg, orally administered) or vehicle (0.5% of carboxymethyl cellulose solution) for another 5 days. One day after the last dose of Olanzapine, the mice were anesthetized and dissected for blood collection by cardiac puncture. Mouse serum was isolated from blood samples by centrifugation. Serum total cholesterol levels were measured using a LabAssay Cholesterol kit (Wako, Osaka, Japan), while serum LDL cholesterol, HDL cholesterol, and triglyceride levels were measured by Nagahama Lifescience (Oriental Yeast Co., Ltd., Shiga, Japan).