A detailed description of blood collection and laboratory measurements has been previously published [10 (link)]. Routine laboratory measurements were performed in fresh serum (basic lipid profile [TC, TG, LDL-C], AST, ALT, CK). The remaining serum was aliquoted and stored at −80°C until assayed for hs-CRP, apoB, Lp(a). All measurements (except for CRP) were performed using the Horiba ABX Pentra 400 analyzer (Horiba ABX, Montpellier, France). LDL-C was measured directly. CRP was measured using the Alinity c analyzer (Abbott Laboratories, Chicago, IL, USA) with the Alinity c CRP Vario High Sensitivity assay for the quantitative, immunoturbidimetric determination of CRP with a limit of detection of 0.4 mg/L. Laboratory measurements were performed at the Department of Laboratory Medicine, Nicolaus Copernicus University, Collegium Medicum, Bydgoszcz, Poland.
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Detailed Blood Collection and Analysis Protocol
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Corresponding Organization :
Other organizations : Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz
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Variable analysis
independent variables
- Not explicitly mentioned
- Total cholesterol (TC)
- Triglycerides (TG)
- LDL-cholesterol (LDL-C)
- Aspartate aminotransferase (AST)
- Alanine aminotransferase (ALT)
- Creatine kinase (CK)
- High-sensitivity C-reactive protein (hs-CRP)
- Apolipoprotein B (apoB)
- Lipoprotein(a) (Lp(a))
- Fresh serum used for measurements
- Aliquoted and stored at -80°C for certain measurements
- Horiba ABX Pentra 400 analyzer used for most measurements
- LDL-C measured directly
- CRP measured using Alinity c analyzer with Alinity c CRP Vario High Sensitivity assay
- Laboratory measurements performed at the Department of Laboratory Medicine, Nicolaus Copernicus University, Collegium Medicum, Bydgoszcz, Poland
- Not explicitly mentioned
- Not explicitly mentioned
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