Biomarker Quantification in Livestock Research

PC and SC levels were analyzed using a SC immunoassay kit according to the specification of kits (Salimetrics LLC, State College, PA, USA). The value for intra- and inter-assay coefficient of variability (CV) of PC was 6.64% and 9.85%, respectively. The intra-assay and inter-assay CV values of SC were 8.84% and 9.13%, respectively. Plasma SP was analyzed using a substance P ELISA kit (Enzo Life Sciences, Farmingdale, NY, USA). The intra-assay CV was 8.42%, whereas the intra-assay CV was 10.6%. Plasma haptoglobin concentrations were analyzed using a bovine haptoglobin enzyme immunoassay kit (GenWay Biotech, San Diego, CA, USA). The intra- and inter-assay CV values were 1.46% and 8.67%, respectively. The analytical methods were verified in our previous study (Park et al [3 (link)]).

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Yoo S., Beak S.H., Kang H.J., Jung D.J., Fassah D.M., Jeong I., Park S.J., Haque M.N., Kim M, & Baik M. (2023). Effect of knife castration on leukocyte cytokine expression and indicators of stress, pain, and inflammation in Korean cattle bull calves. Animal Bioscience, 36(3), 521-528.

Publication 2023

substance P ELISA kit

Assay Bovine Elisa Enzyme immunoassay Haptoglobin Immunoassay Plasma Substance p

Corresponding Organization :

Other organizations : Seoul National University, Pusan National University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

PC levels
SC levels
Plasma SP
Plasma haptoglobin concentrations

control variables

The analytical methods were verified in the authors' previous study (Park et al [3])

controls

Positive control: None mentioned
Negative control: None mentioned

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