Drosophila Genetic Toolkit for Developmental Studies

The w¹¹¹⁸ (used as wild-type strain in this study), dKlf15^NN (CG2932, FBgn0025679; previously known as Bteb2^f06447 was described in the study by Ivy et al^{17 (link)}), Dorothy-Gal4 (dot-Gal4, originally described in the study by Kimbrell et al^{19 (link)}), UAS-mCherry (TRiP control line), dSparc^MI00329 (with an MiMIC insertion in the 5-prime region of the dSparc locus; as described in the study by Venken et al^{20 (link)}), and Tub-Gal80^ts lines were all from the Bloomington Stock Center (Bloomington, IL). The HandC-Gal4 (Hand-Gal4) line was described in the study by Sellin et al^{21 (link)}). The 2 RNAi lines for knocking-down dKlf15 were from the Vienna Drosophila RNAi stock Center (with a targeting hairpin inserted into the second chromosome, VDRC) and Bloomington (Klf15^JF02420, a Harvard TRiP line with a dKlf15 targeting hairpin inserted into the third chromosome). All genetic combinations were generated by standard crosses. Generation of TARGET flies (Hand-Gal4; Tub-Gal80^ts) was achieved by standard crosses.^{22 (link)}