The drift tube ion mobility spectrometer utilized for IMS-MS experiments (Agilent 6560) has been extensively characterized.25 (link),26 (link) Briefly, the instrument consists of a 78 cm uniform field drift tube pressurized with high-purity nitrogen drift gas. Analytes were ionized using the Agilent Jet Stream source in both positive and negative modes. In accordance with the standardized protocol from Stow et al.,27 (link) pressure in the drift tube was maintained at 3.95 Torr and the applied separation voltage was 1574 V. For CID-MS experiments, all ions experienced 10 and 20 V activation in the collision cell (CID) in discrete experiments. No mass selection was utilized in the quadrupole prior to ion activation; all analytes were activated in unison. Fragmentation patterns were matched to reference spectra (both experimental and predicted) as available in METLIN,28 (link) HMDB,29 (link) and Mass Bank of North America (MoNA).30 For extended IMS-MS settings, see Supporting Information Figure S1 .
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Characterization of Drift Tube Ion Mobility Spectrometry
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Corresponding Organization : Washington University in St. Louis
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Variable analysis
independent variables
- Collision energy (10 V and 20 V)
- Fragmentation patterns
- Drift tube length (78 cm)
- Drift gas (high-purity nitrogen)
- Drift tube pressure (3.95 Torr)
- Separation voltage (1574 V)
- Ionization mode (positive and negative)
- No mass selection was utilized in the quadrupole prior to ion activation; all analytes were activated in unison.
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