Three-month-old mice were anesthetized with intraperitoneal ketamine (100 mg/kg; Henry Schein, Melville, NY, USA) and xylazine (10 mg/kg; Henry Schein), and eyes were dilated with 1% tropicamide ophthalmic solution (NDC #17478-102-12; Akorn Inc., Lake Forest, IL, USA). Images were taken using a Micron IV retinal imaging system (Phoenix Research Laboratories, Pleasenton, CA, USA). After in vivo imaging, some animals were allowed to recover on a heating pad, and others were intracardially perfused and eyes enucleated for histological analysis.14 (link),15 After recovery, animals were allowed to rest for at least 1 week before undergoing visual acuity and IOP measurement.
Protocol detail
In Vivo Retinal Imaging of Mice
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Variable analysis
independent variables
- Anesthesia (intraperitoneal ketamine and xylazine)
- Retinal imaging
- Histological analysis
- Visual acuity
- Intraocular pressure (IOP) measurement
- Mouse age (three-month-old)
- Eye dilation (1% tropicamide ophthalmic solution)
- Retinal imaging system (Micron IV)
- Not explicitly mentioned
- Not explicitly mentioned
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