DNA Damage Induction in aNHEJ Reporter Cells

Cells and the EJ2-GFP and MMEJ-GFP aNHEJ reporter cells were cultured as described (8 (link), 9 (link), 41 ). For inducing DNA damage, cells were treated with VP16 from Sigma Aldrich (Cat # E1383-25MG) and Cytosine B-d-arabinofuranoside (Ara-C) from Sigma Aldrich (Cat # C6645).

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Srinivasan G., Williamson E.A., Kong K., Jaiswal A.S., Huang G., Kim H.S., Schärer O., Zhao W., Burma S., Sung P, & Hromas R. (2019). MiR223-3p promotes synthetic lethality in BRCA1-deficient cancers. Proceedings of the National Academy of Sciences of the United States of America, 116(35), 17438-17443.

Publication 2019

VP16

Cells Cytosine Dna damage Vp16

Corresponding Organization :

Other organizations : The University of Texas Health Science Center at San Antonio, Institute for Basic Science, Ulsan National Institute of Science and Technology

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Variable analysis

independent variables

Treatment with VP16 from Sigma Aldrich (Cat # E1383-25MG)
Treatment with Cytosine B-d-arabinofuranoside (Ara-C) from Sigma Aldrich (Cat # C6645)

dependent variables

Outcome measurements in EJ2-GFP and MMEJ-GFP aNHEJ reporter cells

control variables

Cells were cultured as described in the referenced publications (8, 9, 41)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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