The murine colorectal carcinoma cell lines CT26 (ATCC CL-2638) and IEC6 (ATCC CRL-1592) were obtained from the American Type Culture Collection [(ATCC), with authentication by short tandem repeat] and maintained in complete medium as described previously (23 (link)). Cell lines were routinely characterized in the laboratory based on morphology and gene-expression patterns. Cells were confirmed to be free of mycoplasma contamination using a mycoplasma detection kit (Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained with RPMI-1640 medium (Corning Cellgro, Manassas, VA, USA) supplemented with 10% heat-inactivated fetal bovine serum (Corning Cellgro) in a 5% CO2 atmosphere at 37°C.
Surgically resected tumor and normal colon from three individual patients were obtained through the University of Kansas Medical Center Biospecimen Repository.
Surgically resected tumor and normal colon from three individual patients were obtained through the University of Kansas Medical Center Biospecimen Repository.
