IHC staining was performed as previously described 16 (link) by using rat monoclonal anti-CD31 antibody (1:200, Santa Cruz), rabbit polyclonal anti-Ki67 antibody (1:200, Abcam), rabbit monoclonal anti-GFP antibody (1:300, Cell Signaling), mouse monoclonal anti-vimentin antibody (1:100, Santa Cruz), or mouse monoclonal anti-PPARγ2 antibody (1:200, Santa Cruz).
For Oil Red O staining, cells or 10 µm cryosections were fixed by neutral buffered 10% formalin, 30 min at room temperature. After rinsing in 60% isopropanol, cells were stained with Oil Red O (Sigma) at 60˚C for 5 min.
For Oil Red O staining, cells or 10 µm cryosections were fixed by neutral buffered 10% formalin, 30 min at room temperature. After rinsing in 60% isopropanol, cells were stained with Oil Red O (Sigma) at 60˚C for 5 min.
