Western blotting was performed as previously described (58 (link),59 (link)) using the following antibodies: Calreticulin, Cell Signaling 2891; Histone H3, abcam ab1791; U1 snRNP 70, Santa Cruz sc-390899; Pol-II, abcam ab5408; PABPC, abcam ab21060; PABPN, abcam ab75855; GAPDH proteintech 60004-1-Ig; Actin, MP Biomedicals 0869100-CF; Tubulin, Sigma T9026. Western blots were visualized using an Odyssey Fc imaging system (LI-COR).
Free full text: Click here