Parasitemia of Pv-malaria was determined by amplifying the 18S rRNA gene using the 7500 Fast qPCR System (Applied Biosystems, Foster, CA, USA) as described previously [40 (link),41 (link)], and is expressed as number of copies/μL. The primers/probes, qPCR cycling conditions, qPCR efficiency and detection limit are shown in S1 Table. Parasitemia was obtained for only 208 Pv-malaria patients (Fig 1).
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