Recombinant Antibody Production in Yeast

The recombinant antibodies scFv-hERG1 and scDb-hERG1-β1 were produced as described in detail in (Duranti et al., 2018 (link)) and (Duranti et al., 2021a (link); Duranti et al., 2021b (link)), respectively. Both the antibodies are recombinant antibodies encompassing the VH and VL portions of the mAb-hERG1, for the scFv-hERG1 and of two monoclonal antibodies directed against hERG1 (the mAb-hERG1) and β1 (the TS2/16 antibody), for the scDb-hERG1-β1. The two recombinant antibodies are both produced in Pichia Pastoris yeast transfected cells. Supernatant is harvested and purified exploiting the His Tag expressed by both proteins by affinity chromatography using AKTA Pure (Cytiva).

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Santini L., Duranti C., Palandri C., Giammarino L., Musumeci M., Carlucci L., Capitani C., Colasurdo R., Recchia F., Cerbai E., Coppini R, & Arcangeli A. (2023). Cardiac safety assessment of a novel recombinant bispecific antibody targeting the ether-à-go-go related gene 1 (hERG1)-β1 integrin macromolecular complex. Frontiers in Pharmacology, 14, 1237431.

Publication 2023

AKTA Pure

Affinity chromatography Antibodies Antibody Cells Pichia pastoris Proteins Yeast

Corresponding Organization : University of Florence

Other organizations : Scuola Superiore Sant'Anna, University of Siena

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Variable analysis

independent variables

Production of recombinant antibodies scFv-hERG1 and scDb-hERG1-β1 in Pichia Pastoris yeast transfected cells

dependent variables

Purification of the recombinant antibodies by affinity chromatography using AKTA Pure (Cytiva)

control variables

His Tag expressed by both recombinant antibodies

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