Tg(fabp10a:pt-β-catenin) larvae were treated with 0.1% dimethyl sulfoxide (DMSO), 1 µM GW7647 (Cayman Chemical, Ann Arbor, MI), 2 µM K-975 (MedChemExpress, Princeton, NJ), 15 µM Etomoxir (Cayman Chemical, Ann Arbor, MI), 2 µM AG1478 (ApexBio, Houston, TX), or 7 μM CAY10599 (Cayman Chemical, Ann Arbor, MI) for 48 h from 12 or 13 days post-fertilization (dpf). Compounds were refreshed every 24 h. The larvae were fed prior to the drug treatments but fasted during the treatments. They were harvested at 14 or 15 dpf for subsequent analyses.
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