Epitope-based Ebola Vaccine Design

Selection of known T-cell epitopes of Ebola viruses was carried out based on the IEDB—Immune Epitope Database (http://iedb.org) [24 (link)]. Prediction of T-cell epitopes was conducted using TEpredict software [25 (link)]. Design of polyepitope antigens was performed with PolyCTLDesigner [26 (link)]. Genes encoding target immunogens were developed using GeneDesigner software [27 (link)]; a compound of codons was optimized to achieve high expression of genes in human cells. Analysis of amino acid sequences of peptides, evaluating their conservatism, statistical analysis of obtained findings, and graph plotting were executed in statistical analysis environment R (version 3.2; https://www.R-project.org/, Vienna, Austria) [28 ].

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Bazhan S.I., Antonets D.V., Karpenko L.I., Oreshkova S.F., Kaplina O.N., Starostina E.V., Dudko S.G., Fedotova S.A, & Ilyichev A.A. (2019). In silico Designed Ebola Virus T-Cell Multi-Epitope DNA Vaccine Constructions Are Immunogenic in Mice. Vaccines, 7(2), 34.

Publication 2019

R (version 3.2;

A compound Amino acid sequences analysis Cells Codons Ebola viruses Epitope Expression genes Genes Human Immunogens Peptides T cell epitopes

Corresponding Organization : State Research Center of Virology and Biotechnology VECTOR

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Variable analysis

independent variables

Selection of known T-cell epitopes of Ebola viruses based on the IEDB
Prediction of T-cell epitopes using TEpredict software
Design of polyepitope antigens using PolyCTLDesigner
Development of genes encoding target immunogens using GeneDesigner software
Optimization of codon composition to achieve high expression of genes in human cells

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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