Visualizing Amyloid-β Deposition in 5XFAD Mouse Brain

Then, 40-μm brain sections from 8 month old 5XFAD mice were stained with rabbit anti-AβpE3 [Pyro Glu3] antibody (SYSY, 1:200) and 6E10 antibody (BioLegend, 1:1000), as described in [46 (link)]. Immunofluorescent sections were visualized and captured using an Olympus FX 1200 confocal microscope. Representative images represent confocal Z-stacks (12 slices at 1.79-micron step intervals) taken at 40× magnification, and Z-stacks (12 slices at 1.51-micron step intervals) taken at 20× magnification [46 (link)].

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Zagorski K., King O., Hovakimyan A., Petrushina I., Antonyan T., Chailyan G., Ghazaryan M., Hyrc K.L., Chadarevian J.P., Davtyan H., Blurton-Jones M., Cribbs D.H., Agadjanyan M.G, & Ghochikyan A. (2023). Novel Vaccine against Pathological Pyroglutamate-Modified Amyloid Beta for Prevention of Alzheimer’s Disease. International Journal of Molecular Sciences, 24(12), 9797.

Publication 2023

6E10 antibody FX 1200 confocal microscope

Antibody Brain Confocal microscope Immunofluorescent Mice Rabbit

Corresponding Organization : Institute for Molecular Medicine

Other organizations : University of California, Irvine, Hope Center for Neurological Disorders

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Variable analysis

independent variables

Age of 5XFAD mice (8 months)

dependent variables

Immunofluorescent staining of brain sections
Visualization and capture of immunofluorescent sections using an Olympus FX 1200 confocal microscope
Capture of representative confocal Z-stacks at 40× and 20× magnification

control variables

Brain section thickness (40-μm)
Primary antibodies used (rabbit anti-AβpE3 [Pyro Glu3] antibody and 6E10 antibody)
Antibody dilutions (1:200 and 1:1000, respectively)

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