Culturing Three Endometrial Cancer Cell Lines

Three human type 1 EMCA cell lines, HEC-1-A, HEC-1-B and Ishikawa, were used in this study. HEC-1-A (ATCC HTB112) and HEC-1-B (ATCC HTB113) were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). Ishikawa cells were provided by Dr. Jennifer Richer (University of Colorado) [37] (link), [38] (link), [39] (link). HEC-1-A was cultured in McCoy's 5A medium (ATCC, Manassas, VA) supplemented with 10% (v/v) fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA), HEC-1-B in Eagle's minimum essential medium (EMEM) (ATCC, Manassas, VA) supplemented with 10%(v/v) FBS and Ishikawa in Minimal essential medium (MEM) (Life Technologies, Carlsbad, CA) supplemented with 1% non-essential amino acids (NEAA), 6 ng/ml insulin and 5% (v/v) FBS. Antibiotics (10 units/ml of penicillin and 10 µg/ml of streptomycin) were added to all culture media. All cell lines were incubated at 37°C in a humidified atmosphere containing 5% carbon dioxide.

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Tsujiura M., Mazack V., Sudol M., Kaspar H.G., Nash J., Carey D.J, & Gogoi R. (2014). Yes-Associated Protein (YAP) Modulates Oncogenic Features and Radiation Sensitivity in Endometrial Cancer. PLoS ONE, 9(6), e100974.

Publication 2014

HEC-1-A (ATCC HTB112) HEC-1-A McCoy's 5A medium fetal bovine serum (FBS) HEC-1-B

Antibiotics Atmosphere Carbon dioxide Cell lines Cells Culture media Emca Essential amino acids Fetal bovine serum Human Insulin Mccoy 5a medium Penicillin Streptomycin

Corresponding Organization :

Other organizations : Geisinger Medical Center, Icahn School of Medicine at Mount Sinai, Geisinger Wyoming Valley Medical Center

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Variable analysis

independent variables

Cell lines: HEC-1-A, HEC-1-B, and Ishikawa

dependent variables

Not explicitly mentioned

control variables

Culture media used for each cell line (McCoy's 5A, EMEM, and MEM)
Supplementation of culture media (10% FBS, 1% NEAA, 6 ng/ml insulin, 5% FBS)
Antibiotics added to all culture media (10 units/ml of penicillin and 10 µg/ml of streptomycin)
Incubation conditions (37°C, 5% CO2, humidified atmosphere)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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