Adult male C57BL/6J mice weighing 30 gm were purchased from The Jackson Laboratory (Bar Harbor, ME) and acclimatized for a week with free access to food and water. Four groups of mice were studied after acclimatization; 1) control, mice kept on tap water; (2 (link)) BSO, provided with 10 mmol/L BSO in drinking water; (3 (link)) BSO+ML385, mice intraperitoneally implanted with mini-osmotic pumps (Model 1004, Durect Corporation, Cupertino, CA) to dispense ML385 (10 mg/kg bodyweight/day) and supplemented with BSO and (4 (link)) ML385, implanted with ML385 filled mini-osmotic pumps. Bodyweight of mice was recorded at the start and completion of the 8-week treatment.
Osmotic pump implantation was performed as detailed by Chang et al. (21 (link)). Briefly, anesthesia was induced by 3% isoflurane and maintained by 1.5% isoflurane throughout the surgical procedure using (SomnoSuite, Kent Scientific Corp, Torrington, CT, USA) anesthesia machine. A midline incision was made in the lower abdomen and the drug-filled osmotic pump was inserted, delivery portal first, in the peritoneal cavity.
A day before sacrifice, urine samples were collected for 24 hours by keeping the mice in standard metabolic cages. All experiments were performed in compliance with Institutional Animal Care and Use Committee and NIH guidelines.
Osmotic pump implantation was performed as detailed by Chang et al. (21 (link)). Briefly, anesthesia was induced by 3% isoflurane and maintained by 1.5% isoflurane throughout the surgical procedure using (SomnoSuite, Kent Scientific Corp, Torrington, CT, USA) anesthesia machine. A midline incision was made in the lower abdomen and the drug-filled osmotic pump was inserted, delivery portal first, in the peritoneal cavity.
A day before sacrifice, urine samples were collected for 24 hours by keeping the mice in standard metabolic cages. All experiments were performed in compliance with Institutional Animal Care and Use Committee and NIH guidelines.
