Immunofluorescence and Proximity Ligation Assay

Attached cultured cells or 6 µm tissue cryosections were fixed with ice-cold acetone for 10 min and immunostained as described in [16 (link)]. The Abs used for labeling are listed in Table S1. To detect and localize the specific interactions of endogenous proteins, the DuoLinkTM in situ PLA (Olink Bioscience, Uppsala, Sweden) was performed [17 (link)]. All immunofluorescence stains were imaged by confocal laser scanning microscopy (LSM800 or LSM880 fast Airyscan; Carl Zeiss AG, Oberkochen, Germany).

Free full text: Click here

Winkler R., Quaas M., Glasmacher S., Wolfrum U., Thalheim T., Galle J., Krohn K., Magin T.M, & Aust G. (2022). The Adhesion G-Protein-Coupled Receptor GPR115/ADGRF4 Regulates Epidermal Differentiation and Associates with Cytoskeletal KRT1. Cells, 11(19), 3151.

Publication 2022

DuoLinkTM in situ PLA LSM880 fast Airyscan

Acetone Cold Confocal laser scanning microscopy Cryosections Cultured cells Immunofluorescence Proteins Stains Tissue

Corresponding Organization : Leipzig University

Other organizations : Johannes Gutenberg University Mainz

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Localization and interactions of endogenous proteins

control variables

Experimental conditions (e.g., acetone fixation, immunostaining protocol, confocal imaging)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!