Generating Hepatocyte-Stellate Cell Spheroids

For the generation of the cell spheroids, cells were seeded into 96-well round-bottom ultra-low attachment plates (Corning) at 2000 viable cells per well. HepG2/LX-2 24:1 spheroids were grown in minimum essential medium (MEM) supplemented as previously described [17 (link)]. The volume of spheroids was determined using the following formula: 4/3 π r³, where “r” is the mean of the long diameter and short diameter of the spheroid divided by two.

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Schwartz B.E., Rajagopal V., Smith C., Cohick E., Whissell G., Gamboa M., Pai R., Sigova A., Grossman I., Bumcrot D., Sasidharan K., Romeo S., Sehgal A, & Pingitore P. (2020). Discovery and Targeting of the Signaling Controls of PNPLA3 to Effectively Reduce Transcription, Expression, and Function in Pre-Clinical NAFLD/NASH Settings. Cells, 9(10), 2247.

Publication 2020

round-bottom ultra-low attachment plates

Cell spheroids Cells

Corresponding Organization : University of Gothenburg

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Variable analysis

independent variables

Seeding density: 2000 viable cells per well

dependent variables

Volume of spheroids

control variables

Cell line: HepG2/LX-2 24:1 spheroids
Culture medium: Minimum essential medium (MEM) supplemented as previously described [17]
Culture vessel: 96-well round-bottom ultra-low attachment plates (Corning)

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