Imaging hMSCs for SEM Analysis

Samples for SEM analysis were processed as per the established protocol of the lab^{44 (link),45 (link)}. Briefly, hMSCs were cultured and differentiated over cover slips. These samples on coverslips were collected and fixed with Karnovsky fixative (4% paraformaldehyde and 1% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4)) for 6–8 h at 4 °C. Dried samples were mounted over aluminium stubs and sputter-coated with gold prior to imaging with EVO18 scanning electron microscope (Zeiss, Oberkochen, Germany) at 5 KVA in secondary electron imaging mode.

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Singh M., Jain M., Bose S., Halder A., Nag T.C., Dinda A.K, & Mohanty S. (2021). 22(R)-hydroxycholesterol for dopaminergic neuronal specification of MSCs and amelioration of Parkinsonian symptoms in rats. Cell Death Discovery, 7, 13.

Publication 2021

EVO18 scanning electron microscope

Aluminium Buffer Electron Fixative Glutaraldehyde Gold Paraformaldehyde Phosphate Scanning electron microscope

Corresponding Organization :

Other organizations : All India Institute of Medical Sciences

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Variable analysis

independent variables

Differentiation of hMSCs

dependent variables

Morphology of differentiated hMSCs

control variables

Cultured hMSCs on coverslips
Karnovsky fixative (4% paraformaldehyde and 1% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4))
Drying of samples
Mounting of samples on aluminium stubs
Sputter-coating of samples with gold
Imaging with EVO18 scanning electron microscope at 5 KVA in secondary electron imaging mode

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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