Immunofluorescence Staining of XFP-Labeled Neurons
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Corresponding Organization :
Other organizations : Allen Institute for Brain Science, Allen Institute, Howard Hughes Medical Institute, Seattle University, University of Washington
Protocol cited in 45 other protocols
Variable analysis
- Perfusion with 4% paraformaldehyde in 0.1 M PB
- Additional 18 hr brain fixation at 4°C
- Cryoprotection in 20% (w/v) sucrose in PBS at 4°C overnight
- Embedding in OCT
- 25-μm cryosectioning using the tape transfer method
- DAPI staining
- Single-label immunofluorescence with anti-GFP antibody
- Fluorescence imaging of XFP
- Immunofluorescence of GFP
- Room temperature
- PBS buffer
- Triton X-100
- Normal goat serum (NGS)
- Goat anti-rabbit IgG-Alexa Fluor 488
- Not explicitly mentioned
- Not explicitly mentioned
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