Quantitative RT-PCR Analysis of RNA

Total RNA was extracted with TRIZOL (TAKARA, 9109, Japan) and reverse transcribed with the High Capacity cDNA Reverse Transcription kit (ABI, 4368814, USA). Real-time quantitative polymerase chain reaction was performed as reported in a previously published paper [22 (link)], using SYBR Premix (Vazyme, Q331-02, China) according to the manufacturer’s instructions in an ABI StepOnePlus instrument. The data were analyzed via the ΔΔCt method and normalized to 18S mRNA. All primer sequences were designed by Primer3Plus (http://www.primer3plus.com/) and are listed in the Supplementary information.

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Gao Y.S., Qian M.Y., Wei Q.Q., Duan X.B., Wang S.L., Hu H.Y., Liu J., Pan C.Y., Zhang S.Q., Qi L.W., Zhou J.P., Zhang H.B, & Wang L.R. (2019). WZ66, a novel acetyl-CoA carboxylase inhibitor, alleviates nonalcoholic steatohepatitis (NASH) in mice. Acta Pharmacologica Sinica, 41(3), 336-347.

Publication 2019

TRIZOL SYBR Premix

Cdna Mrna Primer Real time polymerase chain reaction Reverse transcription Trizol

Corresponding Organization :

Other organizations : China Pharmaceutical University, Dali University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

MRNA expression levels

control variables

18S mRNA as normalization control

controls

No positive or negative controls were explicitly mentioned.

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