The total RNAs were extracted from cultured cells using TRIzol reagent (Takara Bio Inc., Dalian, China) according to a previously described method [27 (link)]. Six hundred ng of total RNA was reverse-transcribed into first-strand cDNA using the Prime RT Master Mix kit (Takara Bio Inc., Dalian, China). cDNA samples containing an equivalent amount of total RNA were quantified using SYBR Premix EX Taq II (Yeasen Biotech Co., Shanghai, China) with specific primer pairs and subjected to the CFX Connect™ Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA). The specific primer pairs used are listed in Table S3 .
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