Cells were seeded into 24-well plates (Corning, Corning, NY, USA) at the density of 5 × 103 cells/cm2 and were cultivated for 24 h before the application of control/FF-containing media. After 48 h, the cells were harvested, re-suspended in the original culture medium and counted with the Coulter Z2 Counter (Beckman Coulter Inc., Fullerton, CA, USA). For estimation of apoptosis, the cells were harvested, re-suspended in the original medium and subjected to the AnnexinV/propidium iodide staining according to the manufacturer’s protocol (BD Pharmigen, San Diego, CA, USA). Flow cytometric detection of apoptotic cells was performed with the FACSAria FACS system (Becton–Dickinson, Heidelberg, Germany [26 (link)]). At least 5 × 103 cells were analyzed for each condition.
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