Encystation of Entamoeba invadens

E. histolytica (G3 and HM-1:IMSS cl6) were routinely maintained as previously described (44 (link)). E. invadens (IP-1) was routinely maintained in a glass tube filled with 6 ml BI-S-33 (proliferation medium). To induce encystation, 2.5 × 10⁵E. invadens trophozoites were seeded in a Nunc cell culture flask with a solid cap (catalog number [no.] 163371; Thermo Fisher Scientific, Waltham, MA, USA) filled with 56 ml BI-S-33 medium and cultivated at 26°C for 5 days. Trophozoites were harvested from the required numbers of flasks and transferred to encystation medium (37 (link)) at a final concentration of 6 × 10⁵/ml.

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Mi-ichi F., Ikeda K., Tsugawa H., Deloer S., Yoshida H, & Arita M. (2021). Stage-Specific De Novo Synthesis of Very-Long-Chain Dihydroceramides Confers Dormancy to Entamoeba Parasites. mSphere, 6(2), e00174-21.

Publication 2021

Nunc cell culture flask

Cell culture Trophozoites

Corresponding Organization : Keio University

Other organizations : Kazusa DNA Research Institute, RIKEN Center for Sustainable Resource Science

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Variable analysis

independent variables

Culture conditions for E. histolytica
Culture conditions for E. invadens
Encystation induction for E. invadens

dependent variables

Growth and maintenance of E. histolytica
Growth and maintenance of E. invadens
Encystation of E. invadens

control variables

Temperature (26°C) for E. invadens encystation

controls

Positive control: E. histolytica (G3 and HM-1:IMSS cl6) maintained as previously described
Negative control: Not explicitly mentioned

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